Review



silico crispr guide rna selection tool  (Benchling Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Benchling Inc silico crispr guide rna selection tool
    Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT
    Silico Crispr Guide Rna Selection Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+selection+tool/pm39910618-242-10-16?v=Benchling+Inc
    Average 86 stars, based on 1 article reviews
    silico crispr guide rna selection tool - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation."

    Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation.

    Journal: Genome biology

    doi: 10.1186/s13059-025-03483-z

    Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT
    Figure Legend Snippet: Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT

    Techniques Used: Genome Wide, Western Blot, Methylation, Mutagenesis, CRISPR, Activity Assay, Staining



    Similar Products

    silico crispr guide rna selection tool  (Benchling Inc)
    86
    Benchling Inc silico crispr guide rna selection tool
    Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT
    Silico Crispr Guide Rna Selection Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+selection+tool/pm39910618-242-10-16?v=Benchling+Inc
    Average 86 stars, based on 1 article reviews
    silico crispr guide rna selection tool - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier
    grna selection tool  (Benchling Inc)
    90
    Benchling Inc grna selection tool
    Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT
    Grna Selection Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+selection+tool/pm37493629-239-30-34?v=Benchling+Inc
    Average 90 stars, based on 1 article reviews
    grna selection tool - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT

    Journal: Genome biology

    Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation.

    doi: 10.1186/s13059-025-03483-z

    Figure Lengend Snippet: Fig. 1 Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2-deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2-wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2flox/flox parental and Setd2flox/flox; Nsd1−/− MEF cell lines. G Crystal violet staining of Setd2flox/flox and Setd2flox/flox; Nsd1−/− MEF cell lines following treatment with 4-OHT

    Article Snippet: Individual sgRNAs for CRISPRi targeting were selected using an in silico CRISPR guide RNA selection tool (Benchling) with corresponding oligos annealed and subcloned by cohesive-end ligation into a lentiviral mU6-(sp)TRACR guide RNA vector following AarI digestion.

    Techniques: Genome Wide, Western Blot, Methylation, Mutagenesis, CRISPR, Activity Assay, Staining